This is how it usually looks when I work on the new tilesets.
Many many groups with many layers in each.
Each solid square represents 32x32 pixels and is divided
by two into 4x 16x16 pixels by doted lines.
In this object tile it isnt as important to divide down to 16x16.
But with the autotiles(Ground/Floor/walls.. etc) it is very
important to achieve a good edge and getting the patterns
to tile seamless.
Since Rpg Maker VX ACE does not overlap object tiles,
I do make alternative cells for some objekt.
For instance a middle cell of the makeup table has a chair
as an alternative. This way the chair(and or person sitting on chair)
can be removed or put there at a later stage.
I wonder if anyone would be interested in my tilesets
for their own project tho...