Nah, it has nothing to do with honorifics. Ikalgo raised suspicion by calling him "Hagya", rather than "Leol" which is what he changed his name to after becoming independent from the Queen. Other ants still call him by "Hagya" sometimes just to piss him off/mock him to other ants. However, it would be reasonable to assume that Flutter, Leol's direct underling, wouldn't call him by that name, even to other people.
To answer the question though, the dub translates the "-sama" honorific as "lord".